Sciencell 內(nèi)皮細胞培養(yǎng)基ECM是專門為正常人類微血管內(nèi)皮細胞體外培養(yǎng)設計的適于其生長的*培養(yǎng)基。是經(jīng)滅菌的液體培養(yǎng)基，包含必需和非必需氨基酸、維生素、有機和無機化合物、激素、生長因子、微量礦物質(zhì)和低濃度胎牛血清(5%)。該培養(yǎng)基緩沖體系為重碳酸鹽，在含5%CO2的細胞培養(yǎng)箱中平衡后pH值為7.4。該培養(yǎng)基的配方能夠選擇性的促進正常人類微血管內(nèi)皮細胞體外培養(yǎng)中的增殖和生長，并為其達到理想營養(yǎng)平衡狀態(tài)。
Sciencell 內(nèi)皮細胞培養(yǎng)基ECMDescription
Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human microvascular endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.
Components
ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Recommended Medium
ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503)
Product Use
ECM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4°C, the ECGS, the FBS and the P/S solution at -20°C. Protect from light.
Shipping
Gel ice.